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fret melting assay  (Bio-Rad)


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    Bio-Rad fret melting assay
    Fret Melting Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fret+melting+assay/10__1016_slash_j__cej__2024__158199-177-20-26?v=Bio-Rad
    Average 93 stars, based on 16 article reviews
    fret melting assay - by Bioz Stars, 2026-08
    93/100 stars

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    DNA FRET-based melting assays for derivatives 1 – 4 (panels ( A – D ), respectively). The telomeric sequence F21T ( top ) or dsDNA F10T ( bottom ) at 0.2 μM were treated with the tested compounds at concentrations between 1 and 10 μM. DNA renaturing curves are represented.

    Journal: Biology

    Article Title: DNA Interactions and Biological Activity of 2,9-Disubstituted 1,10-Phenanthroline Thiosemicarbazone-Based Ligands and a 4-Phenylthiazole Derivative

    doi: 10.3390/biology13010060

    Figure Lengend Snippet: DNA FRET-based melting assays for derivatives 1 – 4 (panels ( A – D ), respectively). The telomeric sequence F21T ( top ) or dsDNA F10T ( bottom ) at 0.2 μM were treated with the tested compounds at concentrations between 1 and 10 μM. DNA renaturing curves are represented.

    Article Snippet: FRET DNA melting assays were performed in microplate-format on a QuantStudio 6 PRO 0.2 mL, 96-well package (Applied Biosystems, Madrid, Spain).

    Techniques: Sequencing